A Target-Site-Specific Screening System for Antifungal Compounds on Appressorium Formation in Magnaporthe grisea
نویسندگان
چکیده
منابع مشابه
Emerging antifungal azoles and effects on Magnaporthe grisea.
Derivatives of pyrazolo[1,5-a][1, 3, 5]triazine-2,4-dione,pyrazolo[1,5-c][1, 3, 5]thiadiazine-2-one, pyrazolo[3,4-d][1, 3]thiazine-4-one, and pyrazolo[3,4-d][1, 3]thiazine-4-thione were screened for antifungal activity against the causal agent of rice blast disease, Magnaporthe grisea. The compounds were tested at doses ranging from 10 to 200mugml(-1), using the commercial fungicide tricyclazol...
متن کاملcDNA Subtractive Cloning of Genes Expressed during Early Stage of Appressorium Formation by Magnaporthe grisea.
The conidial germ tube of the fungus Magnaporthe grisea differentiates an infection-specific structure, an appressorium, for penetration into the host plant. Formation of the appressorium is also observed on synthetic solid substrata such as polycarbonate. We found that a plant lectin, concanavalin A, specifically suppressed the appressorium formation without affecting the germling adhesion if ...
متن کاملMstu1, an APSES transcription factor, is required for appressorium-mediated infection in Magnaporthe grisea.
The APSES protein family includes important transcriptional regulators of morphological processes in ascomycetes. We identified a deletion mutant of the APSES protein Mstu1 in Magnaporthe grisea that showed reduced conidiation and mycelial growth. Mstu1 formed a number of appressoria comparable to the wild type, although appressorium formation was delayed. In M. grisea, rapid transfer of conidi...
متن کاملMagnaporthe grisea cutinase2 mediates appressorium differentiation and host penetration and is required for full virulence.
The rice blast fungus Magnaporthe grisea infects its host by forming a specialized infection structure, the appressorium, on the plant leaf. The enormous turgor pressure generated within the appressorium drives the emerging penetration peg forcefully through the plant cuticle. Hitherto, the involvement of cutinase(s) in this process has remained unproven. We identified a specific M. grisea cuti...
متن کاملThe Adenylate Cyclase Gene MACI of Magnaporthe grisea Controls Appressorium Formation and Other Aspects of Growth and Development
Magnaporthe grisea, the causal agent of rice blast disease, differentiates a specialized infection structure called an appressorium that is crucial for host plant penetration. Previously, it was found that cAMP regulates appressorium formation. To further understand the cellular mechanisms involved in appressorium formation, we have cloned a gene (MACl) encoding adenylate cyclase, a membrane-bo...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2000
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto.2000.90.10.1162